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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Dec 1, 2025

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

Cell immunocapture microfluidic chip based on high-affinity recombinant protein binders.

Jiří Smejkal¹, Petr Malý², Milan Kuchař²

¹Faculty of Science, University of Jan Evangelista Purkyně in Ústí nad Labem, 400 96, Pasteurova 3544/1, Ústí nad Labem, Czech Republic.

Biosensors & Bioelectronics

|November 8, 2020

Summary

Small recombinant protein binders (PBs) offer a flexible and cost-effective alternative to antibodies for cell immunocapture in microfluidic devices. These PBs achieved significant capture efficiency for specific cells, demonstrating potential for advanced medical diagnostics.

Keywords:

Albumin-binding domain Cell immunocapture Microfluidics Rare cell population Recombinant protein binders

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Area of Science:

Biomedical Engineering
Molecular Biology
Microfluidics

Background:

Cell immunocapture microfluidic devices are crucial for medical diagnostics.
Traditional methods rely on antibody-coated surfaces for cell binding.
Recombinant protein binders (PBs) present a novel alternative with unique advantages.

Purpose of the Study:

To evaluate the efficacy of small recombinant protein binders (PBs) for cell immunocapture in microfluidic devices.
To compare the performance of different PBs targeting specific cell receptors (IL-23R and IL-17RA).
To investigate the impact of PB design and immobilization on cell capture efficiency.

Main Methods:

Utilized recombinant protein binders (REX and ARS ligands) derived from Albumin Binding Domain (ABDwt).
Designed a glass microfluidic Y-system with a planar immunocapture channel operating in a stop-flow dynamic regime.
Tested four PB variants with different epitope specificities and immobilization strategies.

Main Results:

Achieved 60-74% immunocapture efficiency for model THP-1 cells on REX/ARS surfaces.
Demonstrated specific immunocapture of THP-1 cells from a mixture with IL-17RA-negative DU-145 cells.
Observed minimal cell binding on control ABDwt surfaces, validating PB specificity.

Conclusions:

Small recombinant protein binders are effective for cell immunocapture in microfluidic systems.
PB design, including epitope targeting and immobilization, significantly influences capture efficiency.
The stop-flow dynamic regime enhances the effectiveness of cell immunocapture, paving the way for rare cell isolation.