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Related Experiment Video

Updated: Dec 1, 2025

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

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Published on: May 18, 2018

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Lactoferrin Induces Tolerogenic Bone Marrow-Derived Dendritic Cells.

Hui-Won Park1, Sun-Hee Park1, Hyeon-Ju Jo1

  • 1Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon, Korea.

Immune Network
|November 9, 2020
PubMed
Summary

Lactoferrin (LF) transforms mouse bone marrow-derived dendritic cells (BMDCs) into tolerogenic dendritic cells (tDCs). These LF-BMDCs suppress T-cell proliferation and promote regulatory T-cell (Treg) differentiation, offering potential therapeutic benefits.

Keywords:
B7 antigensDendritic cellsImmune toleranceLactoferrinRegulatory T cellsSuppressive factor

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cells (DCs) are key antigen-presenting cells (APCs) that regulate immune responses.
  • Tolerogenic DCs (tDCs) induce immune tolerance by suppressing T-cell activity and promoting regulatory T-cells (Tregs).
  • Lactoferrin (LF) has shown potential in inducing Tregs and protecting against inflammatory bowel disease.

Purpose of the Study:

  • To investigate the tolerogenic effects of lactoferrin (LF) on mouse bone marrow-derived dendritic cells (BMDCs).
  • To elucidate the mechanisms by which LF induces tolerogenic properties in DCs.

Main Methods:

  • BMDCs were treated with LF.
  • Expression of surface molecules (CD80/86, MHC class II) was analyzed.
  • T-cell proliferation and Foxp3+ induced Treg (iTreg) differentiation were assessed in co-cultures.
  • Levels of suppressive molecules (arginase-1, indoleamine 2,3-dioxygenase-1) were measured.
  • Inhibitors of suppressive molecules were used to assess their role.

Main Results:

  • LF treatment reduced CD80/86 and MHC class II expression on BMDCs.
  • LF-BMDCs suppressed T-cell proliferation and enhanced iTreg differentiation.
  • LF-BMDCs showed increased levels of arginase-1 and indoleamine 2,3-dioxygenase-1.
  • The suppressive activity of LF-BMDCs was partially reversed by inhibitors of these enzymes.

Conclusions:

  • Lactoferrin effectively induces tolerogenic properties in dendritic cells.
  • LF-BMDCs suppress T-cell proliferation and promote iTreg differentiation.
  • The mechanism involves downregulation of costimulatory molecules and upregulation of key suppressive enzymes.