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Recent advances in screening active components from natural products based on bioaffinity techniques.

Xiaofang Hou^1,2, Meng Sun^3,2, Tao Bao^1,2

¹School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.

Acta Pharmaceutica Sinica. B

|November 9, 2020

Summary

New bioaffinity screening methods efficiently detect low-concentration active components in natural products. These advanced techniques accelerate drug discovery by reducing time and cost, leading to better therapeutic agents.

Keywords:

AAs, amaryllidaceous alkaloids ABCA1, ATP-binding cassette transporter A1 ACE, affinity capillary electrophoresis APTES, 3-aminopropyl-triethoxysilane ASMS, affinity selection mass spectrometry Active components Bioaffinity techniques CMC, Cell membrane chromatography CMMCNTs, Cell membrane magnetic carbon nanotube CMSP, Cell membrane stationary phase CNT, carbon nanotubes ChE, cholesterol efflux EGFR, epidermal growth factor receptor FP, fluorescence polarization Fe3O4–NH2, aminated magnetic nanoparticles HCS, high content screen HTS, high throughout screen HUVEC, human umbilical vein endothelial cells IMER, immobilized enzyme microreactor MAO-B, monoamine oxidases B MNP, immobilized on nanoparticles MPTS, 3-mercaptopropyl-trimethoxysilane MS, mass spectrometry MSPE, magnetic solid-phase extraction Natural products PD, Parkinson's disease PMG, physcion-8-O-β-d-monoglucoside RGD, arginine-glycine-aspartic acid SPR, surface plasmon resonance STAT3, signal transducer and activator of transcription 3 Screening TCMs, traditional Chinese medicines TYR, tyrosinase TYR-MNPs, tyrosinase-immobilized magnetic nanoparticles Topo I, topoisomerase I UF, affinity ultrafiltration XOD, xanthine oxidase α1A-AR, α1A-adrenergic receptor

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Area of Science:

Natural Product Chemistry
Analytical Chemistry
Drug Discovery

Background:

Natural products are vital sources of drug leads.
Traditional analytical methods struggle to detect low-concentration active compounds in complex natural product mixtures.

Purpose of the Study:

To review technological advances (2015-2019) in separating and screening bioactive components from natural resources.
To highlight emerging bioaffinity-based screening methods.

Main Methods:

Review of recent literature on separation and screening technologies.
Focus on bioaffinity techniques: biological chromatography, affinity electrophoresis, affinity mass spectroscopy, and magnetic/optical methods.

Main Results:

Bioaffinity screening methods offer significant advantages over traditional techniques.
These methods enable direct identification of active components based on target-component affinity.
Tedious separation steps are minimized, saving time and resources.

Conclusions:

Emerging bioaffinity screening tools accelerate the identification of bioactive compounds from natural products.
These advanced methods reduce drug discovery timelines and costs.
The development of more effective and targeted therapeutic agents is facilitated.