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Heme increases peroxidase-antibody activity in aged conjugates
1Laboratorio de Investigación, INVESGEN, Madrid, Spain.
Journal of Immunological Methods
|November 23, 1987
Summary
Heme addition to antibody-peroxidase conjugates, used in clinical kits, stabilizes their activity during storage. This prevents decay and allows for ready-to-use immunoenzymatic assays.
Area of Science:
- Biochemistry
- Immunology
- Clinical Diagnostics
Background:
- Antibody-peroxidase conjugates are crucial reagents in immunoenzymatic assays.
- These conjugates can lose activity over time, especially when stored in diluted forms typical of clinical kits.
Purpose of the Study:
- To investigate methods for stabilizing antibody-peroxidase conjugates.
- To determine if heme can prevent or restore activity loss in these conjugates.
Main Methods:
- Antibody-peroxidase conjugates were diluted and aged at 4°C.
- Activity was measured after storage.
- Heme was added to some samples before use or included in the storage buffer.
Main Results:
- Diluted conjugates showed faster decay of activity.
- Incubation with heme before use partially restored lost activity.
- Including heme in the storage buffer prevented some activity decay.
Conclusions:
- Heme stabilizes antibody-peroxidase conjugates, independent of its pseudoperoxidase activity.
- Heme addition offers a practical method to improve the shelf-life and readiness of conjugates for clinical immunoenzymatic assays.