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Performance of the two-site immunoradiometric assay for serum thyroid-stimulating hormone. Effects of changes in
M G McConway1, E M Biggart, R S Chapman
1Department of Pathological Biochemistry, Royal Infirmary, Glasgow, U.K.
Journal of Immunological Methods
|November 23, 1987
Abstract:
Ten solid-phases were evaluated for their usefulness in a two-site immunoradiometric assay for serum thyroid-stimulating hormone. The criteria used to assess each reagent included the minimum detection limit attainable, the change of binding over the concentration range (signal: noise ratio), and ease of preparation of the reagents. Of all the solid phases tested, Sepharose CL-4B and activated CH-Sepharose 4B showed the characteristics best suited to IRMA, while epoxy-activated Sepharose 6B and Ultrogel ACA-44 were marginally less effective.