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TNK2-AS1 upregulated by YY1 boosts the course of osteosarcoma through targeting miR-4319/WDR1

Weitao Yao1, Qiang Yan1, Xinhui Du1

  • 1Department of Bone and Soft Tumor, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Cancer Science
|November 9, 2020
PubMed

Insights

The long non-coding RNA TNK2-AS1 promotes osteosarcoma (OS) progression by increasing WDR1 expression. Targeting TNK2-AS1 may offer a new therapeutic strategy for OS patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in cancer development.
  • Osteosarcoma (OS) is a primary bone malignancy with significant metastatic potential.
  • The specific functions of lncRNAs in OS progression require further elucidation.

Purpose of the Study:

  • To investigate the role and mechanism of TNK2-AS1 in osteosarcoma (OS).
  • To explore the regulatory relationship between TNK2-AS1, miR-4319, and WDR1 in OS.
  • To identify potential therapeutic targets for OS treatment.

Main Methods:

  • Quantitative real-time PCR to assess TNK2-AS1 expression in OS tissues and cell lines.
  • Cell proliferation, migration, invasion, and apoptosis assays to evaluate TNK2-AS1 function.
  • Western blotting and luciferase reporter assays to confirm molecular interactions.
  • In vivo tumor growth assays in mouse models.

Main Results:

  • TNK2-AS1 expression was significantly upregulated in OS tissues and cell lines.
  • Downregulation of TNK2-AS1 suppressed OS cell proliferation, migration, and invasion, while promoting apoptosis.
  • TNK2-AS1 acted as a molecular sponge for miR-4319, leading to increased WDR1 expression.
  • YY1 transcriptionally activated TNK2-AS1, contributing to OS progression via the TNK2-AS1/miR-4319/WDR1 axis.

Conclusions:

  • TNK2-AS1 promotes osteosarcoma progression by upregulating WDR1 through sponging miR-4319.
  • The YY1/TNK2-AS1/miR-4319/WDR1 pathway is crucial for OS development.
  • TNK2-AS1 represents a potential therapeutic target for osteosarcoma.
Keywords:
TNK2-AS1WDR1YY1miR-4319osteosarcoma

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