Related Experiment Video

Updated: Dec 1, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell

Shun-Lai Li1, Ting-Qi Jiang1, Qing-Wei Cao2

  • 1Department of Urology, The Fifth People's Hospital of Jinan, Jinan, P.R. China.

Journal of Chemotherapy (Florence, Italy)
|November 9, 2020
PubMed

Insights

ADAM29 is overexpressed in clear cell renal cell carcinoma (ccRCC), promoting tumor growth and metastasis. This finding suggests ADAM29 could serve as a prognosticator and therapeutic target for ccRCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • ADAM29 (a disintegrin and metalloproteinase domain) expression is altered in various cancers.
  • Its specific role in clear cell renal cell carcinoma (ccRCC) remains largely uninvestigated.

Purpose of the Study:

  • To elucidate the biological function and mechanism of ADAM29 in ccRCC.
  • To assess ADAM29 as a potential prognostic marker for ccRCC.

Main Methods:

  • Bioinformatic analysis of TCGA ccRCC RNA-Seq dataset.
  • In vitro functional experiments (cell growth, invasion, migration assays).
  • Western blot analysis for protein expression (Cyclin D1, PCNA, MMP9, Snail).

Main Results:

  • ADAM29 is significantly upregulated in ccRCC tissues and cell lines compared to normal controls.
  • High ADAM29 expression correlates with poor prognosis and serves as an independent prognosticator.
  • ADAM29 promotes ccRCC cell proliferation, invasion, and migration.
  • ADAM29 positively correlates with proliferation markers (Cyclin D1, PCNA) and migration markers (MMP9, Snail).

Conclusions:

  • ADAM29 functions as an oncogene in ccRCC.
  • It enhances tumor cell proliferation, invasion, and migration by regulating Cyclin D1/PCNA/MMP9/Snail pathways.
  • ADAM29 presents a promising prognosticator and therapeutic target for ccRCC.
Keywords:
Bioinformatics analysisbiomarkercell viabilityprognosissurvival

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