Green fluorescent protein inspired fluorophores
Jia Kong1, Yuefei Wang2, Wei Qi3
1State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Advances in Colloid and Interface Science
|November 9, 2020
Summary
Green fluorescence proteins (GFP) and GFP-inspired fluorophores offer biocompatible, simple, and versatile bio-organic optical materials for diverse biomedical and biotechnology applications, including advanced imaging and sensing.
Area of Science:
- Biochemistry and Biotechnology
- Materials Science
- Optical Materials
Background:
- Green fluorescence proteins (GFP) are widely utilized in biomedical and biotechnology fields.
- Applications include protein fusion, subcellular localization, cell visualization, protein-protein interaction studies, and genetically encoded sensors.
- Existing GFP mimics include analogs, hydrogen bond-rich proteins, and nanostructures.
Purpose of the Study:
- To summarize the structure and luminescent mechanism of GFP.
- To discuss the design strategies, fluorescent properties, and advanced applications of GFP-inspired fluorophores.
- To highlight the potential of these bioinspired fluorophores as next-generation bio-organic optical materials.
Main Methods:
- Literature review and summary of existing research on GFP and its mimics.
- Analysis of structural and mechanistic aspects of GFP fluorescence.
- Discussion of design principles for GFP-inspired fluorophores based on structure-property relationships.
Main Results:
- Comprehensive overview of GFP structure and luminescence mechanisms.
- Detailed discussion of various GFP-inspired fluorophores, their design, and properties.
- Exploration of advanced applications leveraging the unique characteristics of these bioinspired materials.
Conclusions:
- GFP-inspired fluorophores offer advantages like biocompatibility, structural simplicity, and nanostructure formation.
- These materials show significant potential for diverse applications in bioimaging and biosensing.
- They represent promising candidates for next-generation bio-organic optical materials.
