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Published on: December 30, 2021
MiR-5787 Attenuates Macrophages-Mediated Inflammation by Targeting TLR4/NF-κB in Ischemic Cerebral Infarction
Zhicheng Bao1, Shuguang Zhang2, Xiaoliang Li3,4
1Department of Neurology, Affiliated Kunshan Hospital of Jiangsu University, Suzhou, Jiangsu, 215300, People's Republic of China.
Abstract:
Accumulating studies have suggested the important role of microRNA (miRNA) in ischemic cerebral infarction. However, little is known of the modifying effect of miR-5787, a newly found miRNA, in ischemic cerebral infarction. We aim to elucidate the effect and underlying molecular mechanism of miR-5787 in the pathogenesis of ischemic cerebral infarction. MiR-5787 is demonstrated to be downregulated in peripheral blood mononuclear cells (PBMCs) samples of patients compared with controls, which is negatively associated with inflammatory cytokines of IL-6 and TNF-α in ischemic cerebral infarction. Besides, the expression of miR-5787 is also negatively related to TLR4, which is unregulated in PBMCs of ischemic cerebral infarction patients. Moreover, TLR4 is demonstrated to be a target of miR-5787 by bioinformatics' analysis and the luciferase reporter assay. In addition, miR-5787 can prevent from the proliferation and migration of macrophages, and attenuate LPS/TLR4-mediated inflammatory response via NF-κB in macrophages. MiR-5787 may be a promising biomarker for ischemic cerebral infarction.
Insights
MicroRNA-5787 (miR-5787) is downregulated in ischemic cerebral infarction patients and may serve as a promising biomarker. It regulates inflammation by targeting Toll-like receptor 4 (TLR4).
Area of Science:
- Biomedical Science
- Molecular Biology
- Neuroscience
Background:
- MicroRNAs (miRNAs) play a crucial role in ischemic cerebral infarction.
- The specific role and mechanism of the newly discovered miR-5787 in this condition remain largely unknown.
Purpose of the Study:
- To investigate the effect and underlying molecular mechanism of miR-5787 in the pathogenesis of ischemic cerebral infarction.
Main Methods:
- Analysis of miR-5787 expression in peripheral blood mononuclear cells (PBMCs) from patients and controls.
- Correlation analysis between miR-5787, inflammatory cytokines (IL-6, TNF-α), and Toll-like receptor 4 (TLR4).
- Bioinformatics analysis and luciferase reporter assay to confirm TLR4 as a miR-5787 target.
- Assessment of miR-5787's impact on macrophage proliferation, migration, and inflammatory response via the NF-κB pathway.
Main Results:
- miR-5787 was found to be downregulated in PBMCs of ischemic cerebral infarction patients compared to controls.
- Decreased miR-5787 expression correlated negatively with inflammatory cytokines IL-6 and TNF-α, and with upregulated TLR4.
- TLR4 was confirmed as a direct target of miR-5787.
- miR-5787 inhibited macrophage proliferation and migration, and attenuated LPS/TLR4-mediated inflammation through the NF-κB pathway.
Conclusions:
- miR-5787 plays a protective role in ischemic cerebral infarction by modulating the TLR4/NF-κB inflammatory pathway.
- miR-5787 may serve as a potential therapeutic target and a promising biomarker for ischemic cerebral infarction.
