Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory systems for gene expression control in cyanobacteria.

Applied microbiology and biotechnology·2020
Same author

Regulation of gene expression by the action of a fungal lncRNA on a transactivator.

RNA biology·2019
Same author

A current view on long noncoding RNAs in yeast and filamentous fungi.

Applied microbiology and biotechnology·2018
Same author

A long noncoding RNA promotes cellulase expression in <i>Trichoderma reesei</i>.

Biotechnology for biofuels·2018

Related Experiment Video

Updated: Dec 1, 2025

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System
07:21

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System

Published on: October 20, 2023

1.7K

RNA Characterization in Trichoderma reesei.

Petra Till1

  • 1Christian Doppler laboratory for optimized expression of carbohydrate-active enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria. petra.till@tuwien.ac.at.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2020
PubMed
Summary

This study details RNA characterization methods in Trichoderma reesei, including RNA extraction and end-point identification using rapid amplification of cDNA ends (RACE). The protocols are optimized for analyzing long noncoding RNAs (lncRNAs).

Keywords:
5′/3′ end determinationElectrophoretic mobility shift assayIn vitro footprintingIn vitro synthesisRACERNARNA structureRNA–protein interactionRNase assayTrichoderma reesei

More Related Videos

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
05:29

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference

Published on: November 21, 2023

1.7K
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.8K

Related Experiment Videos

Last Updated: Dec 1, 2025

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System
07:21

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System

Published on: October 20, 2023

1.7K
Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
05:29

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference

Published on: November 21, 2023

1.7K
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.8K

Area of Science:

  • Molecular Biology
  • Mycology
  • Biochemistry

Background:

  • Accurate characterization of RNA molecules is crucial for understanding gene regulation and cellular functions.
  • Trichoderma reesei is a significant fungus with biotechnological applications, necessitating detailed molecular analysis.
  • Specific methods are required for analyzing noncoding RNAs (ncRNAs), particularly long noncoding RNAs (lncRNAs), due to their size and regulatory roles.

Purpose of the Study:

  • To provide a comprehensive overview of established and optimized protocols for RNA characterization in Trichoderma reesei.
  • To detail methods for RNA extraction, end-point identification, in vitro synthesis, and functional analysis.
  • To enable the detailed study of specific RNA species, including long noncoding RNAs (lncRNAs).

Main Methods:

  • Total RNA extraction from fungal mycelia.
  • Rapid Amplification of cDNA Ends (RACE) for identifying 5' and 3' RNA termini.
  • In vitro synthesis and fluorescence labeling of RNA.
  • RNA Electrophoretic Mobility Shift Assays (RNA-EMSA) for analyzing RNA-nucleic acid and RNA-protein interactions.
  • RNA in vitro footprinting for precise mapping of protein-binding sites and RNA structure determination.

Main Results:

  • Established protocols for efficient RNA extraction and precise identification of RNA 5' and 3' ends in Trichoderma reesei.
  • Demonstrated successful in vitro synthesis and labeling of specific RNA molecules.
  • Validated the utility of RNA-EMSA and RNA in vitro footprinting for characterizing RNA-protein interactions and RNA structures.

Conclusions:

  • The presented methods offer a robust toolkit for the comprehensive characterization of RNAs, especially lncRNAs, in Trichoderma reesei.
  • These protocols facilitate the in-depth study of RNA functions, interactions, and structures at a single-nucleotide resolution.
  • The optimized methods contribute to advancing molecular research in fungal systems and ncRNA biology.