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Related Experiment Video

Updated: Dec 1, 2025

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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Analysis and Isolation of Mouse Leukemic Stem Cells.

Fang Dong1, Haitao Bai1, Hideo Ema2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2020
PubMed
Summary

Flow cytometry enables precise analysis and isolation of mouse hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs). These purified cells can be functionally identified and studied at the molecular level for research and clinical applications.

Keywords:
Flow cytometryHematopoietic stem cells (HSCs)ImmunophenotypeLeukemic stem cells (LSCs )

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Area of Science:

  • * Hematology
  • * Immunology
  • * Cancer Biology

Background:

  • * Flow cytometry is a standard technique in basic and clinical research for cell analysis.
  • * Hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs) are critical targets in various diseases.
  • * Previous methods allowed for analysis but lacked detailed protocols for specific isolation of mouse HSCs and LSCs.

Purpose of the Study:

  • * To detail flow cytometry methods for the analysis and isolation of mouse HSCs and LSCs.
  • * To provide a reproducible protocol for researchers studying stem cell biology and leukemia.
  • * To enable functional and molecular studies of purified HSCs and LSCs.

Main Methods:

  • * Utilizing multi-parameter flow cytometry for cell surface marker analysis.
  • * Employing fluorescence-activated cell sorting (FACS) for high-purity isolation of specific cell populations.
  • * Implementing established protocols for mouse bone marrow sample preparation.

Main Results:

  • * Successful high-purity isolation of mouse HSCs and LSCs was achieved using the described flow cytometry methods.
  • * The isolated cells were demonstrated to be viable and suitable for downstream applications.
  • * Characterization confirmed the identity and purity of the sorted cell populations.

Conclusions:

  • * Flow cytometry provides a powerful tool for the precise analysis and isolation of mouse HSCs and LSCs.
  • * The described methods facilitate further functional and molecular investigations of these critical cell types.
  • * This protocol supports advancements in understanding stem cell biology and developing new therapeutic strategies for leukemia.