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Updated: Dec 1, 2025

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Published on: April 16, 2013
Isolation, Culture, and Manipulation of Human Cord Blood Progenitors
Cristina Prieto1,2, Damia Romero-Moya3, Rosa Montes4
1VIB Center for Cancer Biology, Leuven, Belgium. cristina.prieto@kuleuven.vib.be.
Abstract:
Umbilical Cord Blood (CB) is a rich source of hematopoietic stem/progenitor cells (HSPCs) with high proliferative capacity and a naïve immune status. These characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vitro assays in order to study leukemic processes.
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