Related Experiment Video
Updated: Dec 1, 2025
08:52
Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
Published on: June 13, 2018
9.1K
Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells
André G Deslauriers1,2,3,4,5,6, Andriana G Kotini1,2,3,4, Eirini P Papapetrou7,8,9,10
1Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2020
Summary
Patient-derived induced pluripotent stem cells (iPSCs) offer a novel method for modeling acute myeloid leukemia (AML). These iPSCs can be differentiated into leukemia stem cells and blasts, aiding in myeloid malignancy research.
Area of Science:
- Stem cell biology
- Hematology
- Cancer research
Background:
- Patient-derived induced pluripotent stem cells (iPSCs) are emerging as a powerful tool for disease modeling.
- Acute myeloid leukemia (AML) and other myeloid malignancies require innovative research models.
Purpose of the Study:
- To establish methods for generating patient-derived iPSCs from leukemia cells.
- To develop protocols for directed in vitro differentiation of these iPSCs into relevant hematopoietic cells.
- To create a model system that recapitulates key features of leukemia stem cells (LSCs) and leukemic blasts.
Main Methods:
- Isolation of leukemia cells for reprogramming into iPSCs.
- Directed differentiation of patient-derived iPSCs into hematopoietic lineages.
- Characterization of differentiated cells for leukemia-specific features.
Main Results:
- Successful generation of patient-derived iPSCs from leukemia samples.
- Achieved directed differentiation of iPSCs into hematopoietic cells.
- Differentiated cells exhibited characteristics of LSCs and leukemic blasts.
Conclusions:
- Patient-derived iPSCs provide a viable platform for modeling AML.
- This approach facilitates the study of leukemia stem cell biology and leukemic blast formation in vitro.
- The developed methods support advancements in understanding and potentially treating myeloid malignancies.
Related Concept Videos
01:21EPS and iPS Cells in Disease Research
3.2K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.2K
01:13Induced Pluripotent Stem Cells
26.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
26.0K
01:06Induced Pluripotent Stem Cells
5.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
5.0K
01:22iPS Cell Differentiation
2.9K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.9K
01:17Stem Cell Culture
5.9K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.9K
01:21Stem Cell Therapy for Tissue Regeneration
4.4K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.4K