Miniaturized Electrochemiluminescence Platform With Laser-Induced Graphene Electrodes for Multiple Biosensing

Area of Science:

• Electrochemistry
• Materials Science
• Sensor Technology

Background:

• Miniaturized sensing platforms are crucial for point-of-care diagnostics and environmental monitoring.
• Laser-Induced Graphene (LIG) offers a rapid and cost-effective method for creating conductive electrode materials.
• Open Bipolar Electrodes (OBEs) provide a unique configuration for electrochemical measurements.

Purpose of the Study:

• To develop a miniaturized, 3D-printed electrochemiluminescence (ECL) sensing platform.
• To utilize Laser-Induced Graphene (LIG) based Open Bipolar Electrodes (OBEs) for enhanced sensing capabilities.
• To integrate smartphone control for driving voltage and sensing ECL signals, enabling a low-cost and accessible system.

Main Methods:

• Fabrication of OBEs on a polyimide (PI) substrate using a CO2 laser for LIG creation.
• Development of a 3D-printed sensing platform integrating the LIG-OBEs.
• Utilizing an Android smartphone for system control, voltage application, and ECL signal detection.
• Optimization of parameters for sensitive detection of analytes.

Main Results:

• Successful detection of Hydrogen Peroxide (H2O2) with a wide linear range and low limit of detection (LOD).
• Accurate quantification of glucose within a specified linear range and a low LOD.
• Demonstrated workability of the platform through testing with real-world samples.
• Achieved high correlation coefficients (R2) for both H2O2 and glucose detection.

Conclusions:

• The developed 3D-printed LIG-OBE platform offers a low-cost, rapid, and miniaturized solution for ECL sensing.
• The smartphone integration makes the system highly accessible for various applications.
• The platform shows significant potential for biomedical applications, environmental monitoring, and point-of-care testing.