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Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
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Processing methods and storage duration impact extracellular vesicle counts in red blood cell units
Clementine Gamonet1, Maxime Desmarets1,2, Guillaume Mourey1,3
1University Bourgogne Franche-Comté, INSERM, Etablissement Français du sang Bourgogne Franche Comté, Unité Mixte de Recherche 1098, Interactions Hôte-Greffon-Tumeur, Ingénierie Cellulaire et Génique, Besançon, France.
Blood Advances
|November 9, 2020
Summary
Red blood cell (RBC) processing methods significantly impact extracellular vesicle (EV) levels. Standardizing these methods could control EV content in transfused RBC units, potentially improving transfusion safety and efficacy.
Area of Science:
- Blood banking and transfusion medicine
- Cellular and molecular biology
- Biotechnology and biomaterials
Background:
- Extracellular vesicles (EVs) are present in red blood cell (RBC) concentrates and may influence transfusion outcomes.
- Understanding factors affecting EV release is crucial for managing RBC unit quality.
Purpose of the Study:
- To investigate the impact of donor characteristics, processing methods, and storage duration on EV counts and characteristics in RBC units.
- To identify independent factors associated with erythrocyte-derived EV (EEV) and platelet-derived EV (PEV) counts.
Main Methods:
- A standardized cytometry-based method was used to quantify EEVs and PEVs in 1226 RBC units.
- EV size and CD47 and annexin V expression were measured.
- Multivariate analysis (including analysis of covariance) was employed to determine independent factors affecting EV counts.
Main Results:
- Whole-blood-filtered RBC units had higher EEV and PEV counts than RBC-filtered units.
- PEV counts correlated with filter type and residual platelet count.
- EEV counts were independently associated with filter type, preparation, and storage time.
- CD47 expression on EEVs increased with longer storage duration.
Conclusions:
- RBC processing methods significantly influence EV quantity and characteristics, leading to variability in transfused EVs.
- Standardization of processing methods may offer a way to control EV content in RBC units.
- Further research is needed to confirm the clinical impact of EVs on patient outcomes.
