Performance and modelling of retention in microemulsion liquid chromatography
N Pankajkumar-Patel1, E Peris-García1, O Schiopu1
1Department of Analytical Chemistry, University of Valencia, c/Dr. Moliner 50, Burjassot, Spain.
Journal of Chromatography. A
|November 9, 2020
Summary
Microemulsion liquid chromatography effectively analyzes parabens and beta-blockers. A new model accurately predicts retention times, revealing how oil in microemulsions impacts solute interactions.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Microemulsions (MEs) offer unique properties as mobile phases in liquid chromatography.
- Understanding ME behavior is crucial for developing advanced separation techniques.
Purpose of the Study:
- To investigate the use of microemulsions as mobile phases for analyzing parabens and beta-adrenoceptor antagonists.
- To develop a mathematical model for predicting retention behavior in microemulsion liquid chromatography.
Main Methods:
- Microemulsions were formulated using sodium dodecyl sulphate, 1-butanol, and octane.
- Stability of MEs was determined through preliminary composition studies.
- Retention behavior, peak profile, and resolution were evaluated with and without octane.
- A global mathematical model was developed to predict retention times.
Main Results:
- Stable microemulsions were successfully formed and characterized.
- The addition of octane to micellar mobile phases influenced retention behavior, peak profile, and resolution.
- A predictive model accurately estimated retention times across various mobile phase compositions (1.1–2.5% error).
- The model indicated that octane modifies solute-micelle interactions within the microemulsion.
Conclusions:
- Microemulsion liquid chromatography is a viable technique for analyzing parabens and beta-blockers.
- The developed mathematical model provides accurate retention time predictions.
- Octane plays a significant role in modulating solute interactions within microemulsion mobile phases.
Keywords:
Micellar liquid chromatographyOil-in-water microemulsion liquid chromatographyParabensRetention modellingβ-Adrenoceptor antagonistsMore Related Videos
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