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The chicken c-fos gene: cloning and nucleotide sequence analysis
K T Fujiwara1, K Ashida, H Nishina
1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Journal of Virology
|December 1, 1987
Summary
Researchers cloned and sequenced the chicken c-fos gene, finding high homology to mouse and human counterparts. This avian fos gene may offer insights into oncogene regulation and expression across species.
Area of Science:
- Molecular Biology
- Genetics
- Oncogenes
Background:
- The fos gene is crucial in cell growth and differentiation.
- Understanding avian c-fos provides comparative insights into mammalian oncogenes.
Purpose of the Study:
- To clone and sequence the chicken c-fos gene.
- To analyze its structural and potential functional similarities to mammalian c-fos genes.
Main Methods:
- Utilized a DNA probe from avian transforming virus NK24 containing the fos gene.
- Cloned and sequenced the chicken c-fos gene.
- Performed sequence analysis and homology comparisons with mouse and human c-fos.
Main Results:
- The chicken c-fos gene has four exons, predicting a 367-amino-acid product.
- Predicted protein shows 79% homology to mouse p55c-fos, with some nonhomologous regions.
- High guanine-cytosine content (93%) observed in the third codon position.
- Homologous sequences found in the 5'-flanking and 3'-noncoding regions compared to mouse/human c-fos.
Conclusions:
- Chicken c-fos shares significant structural and regulatory sequence homology with mammalian c-fos.
- The avian c-fos gene's conserved 3'-noncoding region suggests a similar role in gene expression regulation.
- This study provides a foundation for further research into avian oncogene function and evolution.