Improving efficiency of measuring individual ¹H coupling networks by pure shift 2D J-resolved NMR spectroscopy

The ¹H coupling networks, including ¹H-¹H correlation and J coupling values, provide the important information for structure elucidation and conformation analysis. However, the presence of a large number of couplings and the phase-twist lineshapes often prevents revealing ¹H coupling networks. Here, we provide a clean absorption-mode 2D NMR method, SIMAJ (SImple Methods for 2D Absorption mode J-resolved spectrum), for a straightforward assignment and measurement of the coupling network involving the chosen proton. Relying on the pure shift element, ¹H-¹H couplings and chemical shift evolution are totally separately demonstrating along the F₁ and F₂ dimensions, respectively. Processing with a single experiment dataset and free of 45° spectral shearing, an absorption-mode 2D J-resolved spectrum can be reconstructed. Two pulse sequences were proposed as examples. The SIMAJ signal processing method will be a general procedure for obtaining absorption-mode lineshapes when analyzing the experiment datasets with chemical shifts and J coupling multiplets in the orthogonal dimensions. With excellent sensitivity, high spectral purity, and ability of easily identifying ¹H-¹H correlations, significant improvements are beneficial for structural, conformational, or complex composition analyses.