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Published on: March 30, 2019
[Over-expression of miR-519d alters gene expression profiles of cervical cancer SiHa cells]
Ye Zeng1, Jie Liu2, Zhichao Cheng3
1Department of Stomatology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Objective:
To investigate the alterations in gene expression profiles of cervical cancer cell line SiHa over-expressing miR-519d.
Methods:
SiHa cells were transfected with a miR-519d mimic or a negative control (NC) and the changes in gene expression profiles were examined using NimbleGen human gene expression microarray. Bioinformatics approaches based on the microarray data were used to identify the targeted genes of miR-519d. Real-time quantitative PCR was employed to confirm the expression of the potential target genes.
Results:
A total of 5172 genes were found to be differentially expressed in SiHa cells over-expressing miR-519d, including 2476 up-regulated and 2796 down-regulated genes. We identified 164 potential target genes of miR-519d, and their functions were predicted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis, and STRING database and pSTIING were used to search the key nodes in the protein-protein interactions and transcriptional regulatory networks in cancer. Real-time quantitative PCR confirmed the differential expressions of several candidate target genes.
Conclusions:
Over-expression of miR-519d alters gene expression profiles in SiHa cells. The 164 target genes of miR-519d we identified may provide insights into the role of miR-519d in cervical tumorigenesis.
Insights
Over-expressing miR-519d significantly alters gene expression in cervical cancer cells (SiHa). Researchers identified 164 potential target genes, offering new insights into cervical cancer development.
Area of Science:
- Molecular Biology
- Genomics
- Cancer Research
Context:
- Cervical cancer is a significant global health concern.
- MicroRNAs (miRNAs) play crucial roles in gene regulation and cancer progression.
- Understanding miRNA targets is vital for developing novel therapeutic strategies.
Purpose:
- To investigate the impact of miR-519d over-expression on gene expression profiles in the SiHa cervical cancer cell line.
- To identify and functionally characterize the target genes regulated by miR-519d.
- To explore the potential role of miR-519d in cervical tumorigenesis.
Summary:
- Gene expression profiling of SiHa cells with over-expressed miR-519d revealed significant alterations, with 2476 genes up-regulated and 2796 down-regulated.
- Bioinformatic analysis identified 164 potential target genes for miR-519d.
- Functional prediction using Gene Ontology and KEGG pathway analysis, along with network analysis, provided insights into the biological roles of these targets in cancer.
Impact:
- This study elucidates the comprehensive gene expression changes induced by miR-519d in cervical cancer cells.
- The identified 164 miR-519d target genes provide a valuable resource for further research into the mechanisms of cervical cancer.
- These findings may contribute to the development of miRNA-based diagnostics and therapeutics for cervical cancer.
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