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Related Experiment Video

Updated: Dec 1, 2025

Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays
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Fiji plugin for annotating movies with custom arrows.

Stephan Daetwyler1, Carl D Modes2,3,4, Reto Fiolka5,6

  • 1Department of Cell Biology, UT Southwestern Medical Center, Dallas, 75390, Texas, USA Stephan.Daetwyler@UTSouthwestern.edu.

Biology Open
|November 10, 2020
PubMed
Summary

This study introduces a user-friendly Fiji plugin for annotating time-lapse microscopy videos. The tool simplifies highlighting dynamic cellular processes using arrows and circles, aiding biological research.

Keywords:
AnnotationArrowArrowheadFiji pluginMovieTime-lapse data

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Area of Science:

  • Microscopy and Image Analysis
  • Cell Biology
  • Bioimage Informatics

Background:

  • Time-lapse microscopy is crucial for observing dynamic biological processes.
  • Manual annotation of microscopy videos is time-consuming and can be subjective.
  • Accurate annotation aids in quantifying cellular behavior and understanding biological mechanisms.

Purpose of the Study:

  • To develop a user-friendly Fiji plugin for annotating time-lapse microscopy data.
  • To enable efficient highlighting of specific cellular events using graphical elements.
  • To facilitate the saving and loading of annotated tracks for reproducible analysis.

Main Methods:

  • Development of a Fiji plugin integrating annotation tools.
  • Implementation of features for adding arrows, arrowheads, and circles to video frames.
  • Inclusion of functionality for saving and retrieving annotation data.

Main Results:

  • A novel Fiji plugin was created for annotating time-lapse movies.
  • The plugin supports dynamic annotation with arrows, arrowheads, and circles.
  • Users can save and load their annotations, improving workflow efficiency.

Conclusions:

  • The developed Fiji plugin offers a streamlined approach to annotating dynamic biological processes in microscopy videos.
  • This tool enhances the analysis of time-lapse data by simplifying the visualization and tracking of cellular events.
  • The plugin promotes reproducibility and efficiency in bioimage analysis workflows.