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Related Experiment Videos

Antigenic expression by cells derived from human gliomas does not correlate with morphological classification.

P G Kennedy1, B A Watkins, D G Thomas

  • 1Institute of Neurology, Queen Square, London, UK.

Neuropathology and Applied Neurobiology
|September 1, 1987
PubMed
Summary

Human glioma cells show unexpected antigenic classes, challenging traditional tumor classification. Many tumor cells express fibronectin, indicating transformation and a departure from glial cell markers.

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Area of Science:

  • Neuroscience
  • Oncology
  • Cell Biology

Background:

  • Traditional neuropathological methods classify gliomas based on morphology and lineage.
  • Established glial-specific markers like glial fibrillary acidic protein (GFAP) are not consistently expressed in glioma cell cultures.
  • Cell-surface fibronectin is typically found on extracellular matrix, not CNS macroglia.

Purpose of the Study:

  • To investigate the antigenic profiles of human glioma-derived cell populations.
  • To determine if antigenic classes correlate with standard morphological classifications or traditional neuropathological assignments.
  • To identify novel antigenic phenotypes in gliomas.

Main Methods:

  • Derivation and culture of cell populations from human gliomas.

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  • Immunohistochemical analysis of cell-surface and intracellular antigens, including GFAP and fibronectin.
  • Examination of antigen expression in tumor biopsies.
  • Assessment of cellular characteristics such as aneuploidy and contact inhibition.

    • Main Results:

    • Glioma-derived cell populations exhibit antigenic classes unpredictable by morphology, frequently not supporting traditional lineage assignments.
    • Low expression of GFAP in astrocytoma cultures, with some GFAP-expressing cultures originating from non-GFAP expressing morphological categories.
    • Absence of oligodendrocyte-specific antigens in oligodendrogliomas and oligo-astrocytomas.
    • Widespread expression of cell-surface fibronectin in most glioma-derived populations across tumor types and grades.
    • Fibronectin-expressing cells in vitro and in situ demonstrated aneuploidy and lack of contact inhibition, indicative of transformation.
    • Identification of two novel antigenic phenotypes within gliomas.

    Conclusions:

    • Antigenic profiling provides a novel classification for human gliomas, diverging from morphological and traditional neuropathological assessments.
    • The frequent expression of fibronectin in glioma cells suggests a transformed phenotype and potential dedifferentiation.
    • Further research into these novel antigenic phenotypes may reveal new diagnostic or therapeutic targets for gliomas.