Cortical direct current stimulation improves signal transmission between the motor cortices of rats

Transcranial direct current (DC) stimulation is a noninvasive brain stimulation technique that is now widely used to improve motor and cognitive function. The neuromodulatory effects of DC is considered to extend to nearby as well as remote brain areas from the site of stimulation because of current flowing into the brain and/or signal transmission in neuronal networks. However, the effects of DC on cortico-cortical neuronal transmission are not well known. In the present study, we focused on signal transmission from the primary (M1) to secondary (M2) motor cortex of rats. Intra-cortical microstimulation (ICMS) was applied to the M1 under DC conditions, and changes in synaptic activity in the M2 were examined using current-source density analyses. The synaptic input to the M2 superficial layers was enhanced during DC stimulation, while the synaptic input to the M2 deeper layers was increased after DC stimulation. These results suggest that DC stimulation improves cortico-cortical neuronal transmission from M1 to M2, and that the effectiveness of DC may be different among different projection neuron types in the M1.