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Surface-assisted laser desorption/ionization mass spectrometry imaging: A review
Wendy H Müller1, Alexandre Verdin1, Edwin De Pauw1
1Mass Spectrometry Laboratory, MolSys Research Unit, Chemistry Department, University of Liège, Liège, Belgium.
Mass Spectrometry Reviews
|November 11, 2020
Summary
Surface-assisted laser desorption/ionization mass spectrometry imaging (SALDI-MSI) offers targeted analysis capabilities. This review explores SALDI-MSI strategies, sample preparation, nanosubstrate selection, and applications in various scientific fields.
Area of Science:
- Analytical Chemistry
- Spectrometry
Background:
- Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) utilizes nanostructured substrates for analyte desorption/ionization.
- While known for untargeted small molecule analysis, SALDI-MS also enables targeted approaches.
Purpose of the Study:
- This review focuses on the implementation and analytical strategies of SALDI-MS imaging (SALDI-MSI).
- It aims to discuss fundamental mechanisms, sample preparation, nanosubstrate selection, and functionalization for targeted SALDI-MSI.
Main Methods:
- Discussion of SALDI-MSI nomenclature and controversial fundamental mechanisms.
- Extensive review of analytical strategies, including sample preparation and nanosubstrate selection (composition, morphology, functionalization).
Main Results:
- Exploration of targeted SALDI-MSI approaches for selective compound analysis.
- Presentation of selected SALDI-MSI applications across biomedical, biological, environmental, and forensic fields.
Conclusions:
- SALDI-MSI presents significant strengths and remaining limitations.
- The technique shows a bright future with proposed perspectives for further development.
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