Micromonospora fluminis sp. nov., isolated from mountain river sediment

During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38^T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38^T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC_15:0, 10 methyl C_17:0, anteiso-C_17:0 and iso-C_17:0. The predominant menaquinones were MK-10(H₄) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142^T (99.5 %), Micromonospora citrea DSM 43903^T (99.4 %), Micromonospora marina DSM 45555^T (99.4 %), Micromonospora maritima DSM 45782^T (99.3 %), Micromonospora sediminicola DSM 45794^T (99.3 %), Micromonospora aurantiaca DSM 43813^T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246^T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026^T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38^T as a novel species. On the basis of the results presented here, we propose to classify strain A38^T (=LMG 30467^T=CECT 30034^T) as the type strain of the novel species Micromonospora fluminis sp. nov.