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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Dec 1, 2025

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Flow Cytometry and Sorting in Arabidopsis.

David W Galbraith^1,2, Guiling Sun³

¹University of Arizona, School of Plant Sciences and Bio5 Institute, Tucson, AZ, USA. galbraith@arizona.edu.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2020

Summary

Flow cytometry and sorting techniques are advancing plant science and agriculture by enabling detailed cellular analysis in model plants like Arabidopsis thaliana. This method allows for in vivo labeling, cell sorting, and transcriptome analysis for deeper biological insights.

Keywords:

Agnostic sampling Flow cytometry Fluorescent proteins Gene expression Next-generation sequencing Nucleus Protoplasts Sorting Spectral analysis Transcription

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Isolation and Transcriptome Analysis of Plant Cell Types

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Last Updated: Dec 1, 2025

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

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Published on: October 4, 2012

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

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Published on: May 29, 2010

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Area of Science:

Plant biology
Cellular analysis
Agricultural science

Background:

Flow cytometry and sorting are established techniques for cellular population analysis.
Applications in higher plants, crucial for biosciences and agriculture, have grown over 40 years.
Plant cells present unique challenges due to cell walls, tissue complexity, and larger size compared to mammalian cells.

Purpose of the Study:

To update and detail the application of flow cytometry and sorting techniques in the model plant Arabidopsis thaliana.
To highlight advancements in in vivo fluorescence labeling, cell type identification, and subcellular component analysis.
To focus on new experimental methods for analyzing sorted plant protoplasts and nuclei, including transcriptome analysis.

Main Methods:

In vivo fluorescence labeling of specific cell types and subcellular components.
Analysis using conventional cytometers and spectral analyzers.
Fluorescence-activated sorting of protoplasts and nuclei for downstream analyses.
Transcriptome analysis of sorted single protoplasts and nuclei.

Main Results:

Successful application of flow cytometry and sorting to Arabidopsis thaliana.
Demonstration of in vivo labeling for precise cell identification.
Effective sorting of protoplasts and nuclei for detailed analysis.
Generation of transcriptome data from sorted single cells.

Conclusions:

Flow cytometry and sorting are powerful tools for advancing plant biosciences and agricultural productivity.
New methods enhance the ability to study cellular interactions within complex plant tissues.
This approach provides unique insights into plant biology at the single-cell level.