Identification and Characterization of Reproductive Mutations in Arabidopsis

Mutations in numerous genes affect reproduction in Arabidopsis leading to sterility and abortion of seed development, respectively. These include mutations in regulators of reproductive development and fertilization, but also in house-keeping genes lacking mutant phenotypes during vegetative development. However, during the haploid phase of germline development or during seed development, lethality or failures become visible when gene activity is needed. Plant reproduction is complex and includes many processes from flowering and flower organ development toward the formation of seeds after a double fertilization process. For those who are less familiar with the various reproductive processes in Arabidopsis and who aim to study the cause of reproductive defects during germline development and function, fertilization, or embryogenesis in a given mutant, we provide here a step-by-step guideline and basic protocols to elucidate the reproductive process affected.