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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Immunoprecipitation01:20

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Dec 1, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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RNA-Binding Protein Immunoprecipitation and High-Throughput Sequencing.

Tino Köster1, Dorothee Staiger2

  • 1Faculty of Biology, RNA Biology and Molecular Physiology, Bielefeld University, Bielefeld, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2020
PubMed
Summary

Researchers developed a new method to identify all RNA targets of RNA-binding proteins in plants. This technique uses RNA immunoprecipitation and high-throughput sequencing for a global analysis of posttranscriptional networks.

Keywords:
Cross-linkingPosttranscriptionalRIP-seqRNA immunoprecipitationRNA-binding protein

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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
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Last Updated: Dec 1, 2025

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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • RNA-binding proteins (RBPs) regulate RNA molecule lifecycle.
  • RBPs coordinate gene processing via sequence-specific interactions.
  • Identifying RBP targets is crucial for understanding posttranscriptional gene regulation.

Purpose of the Study:

  • To develop a method for comprehensive identification of in vivo RNA targets for RBPs.
  • To enable global-scale analysis of RNA-RBP interactions in plants.

Main Methods:

  • RNA immunoprecipitation (RIP) coupled with library preparation for high-throughput sequencing.
  • Application of the protocol in Arabidopsis thaliana.

Main Results:

  • The protocol allows for the determination of RNAs associated with RBPs.
  • This method enables a global assessment of RNA-RBP interactions in planta.

Conclusions:

  • The described protocol provides a powerful tool for global identification of RBP targets.
  • This advancement facilitates a deeper understanding of posttranscriptional regulatory networks in plants.