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Updated: Dec 1, 2025
10:38
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
Published on: September 21, 2018
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Overview of the centriole architecture
Maeva LeGuennec1, Nikolai Klena1, Gabriel Aeschlimann2
1University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland.
Current Opinion in Structural Biology
|November 11, 2020
Summary
The centriole, a large cell component, has its overall structure visualized using advanced imaging. This review details its organization, sub-regions, and protein makeup, despite its atomic structure remaining unknown.
Area of Science:
- Cell Biology
- Structural Biology
- Molecular Assembly
Background:
- The centriole is a massive molecular assembly within eukaryotic cells, crucial for various cellular functions.
- Despite its significance, the complete atomic structure of the centriole remains elusive.
- Existing knowledge relies on various imaging techniques providing a global architectural perspective.
Purpose of the Study:
- To review the current understanding of the centriole's overall organization.
- To highlight key sub-structural elements and their variations across species.
- To discuss the putative protein composition of centriole sub-structures.
Main Methods:
- Electron microscopy
- Cryo-electron tomography
- Super-resolution microscopy
Main Results:
- Establishment of a global architectural view of the centriole and its sub-regions.
- Identification of differences in centriole structure between species.
- Proposals for the protein composition of various centriole sub-structures.
Conclusions:
- Current imaging techniques provide significant insights into centriole architecture.
- A complete atomic-level understanding of the centriole is still a future goal.
- Further research is needed to bridge the gap towards an atomic view.
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