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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Risk factors for Keshan disease: a prospective cohort study protocol of gut flora
Zhenzhen Li1,2, Jin Wei3,2, Yanping Zhang1,2
1Department of Clinical Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, 710004, People's Republic of China.
Insights
This study investigates gut flora in Keshan disease, aiming to find early diagnostic biomarkers and new therapeutic targets. By analyzing gut microbiome data, researchers seek to understand the disease
Area of Science:
- Cardiovascular Research
- Microbiome Research
- Genomics and Bioinformatics
Background:
- Keshan disease is an endemic cardiomyopathy with unknown causes, hindering diagnosis and treatment.
- Understanding the gut microbiome's role in cardiovascular diseases offers potential for new diagnostic and therapeutic strategies.
- This study focuses on identifying gut flora biomarkers for early diagnosis and therapeutic targets in Keshan disease.
Purpose of the Study:
- To identify novel biomarkers for the early diagnosis of Keshan disease.
- To explore new therapeutic targets for Keshan disease by investigating the gut microbiome.
- To elucidate the relationship between gut flora and host in Keshan disease patients.
Main Methods:
- A cohort study involving sample collection (blood, stool) and clinical data (ECG, echocardiogram) from participants.
- Prospective follow-up over 2 years with data collected every 6 months.
- Deep data mining, machine learning, metagenomics, and metabonomics for comprehensive analysis.
Main Results:
- The study will clarify gut flora distribution and changes in different stages of Keshan disease.
- It aims to identify key environmental and clinical factors influencing Keshan disease through big data analysis.
- Specific gut flora and molecular pathways implicated in Keshan disease will be identified.
Conclusions:
- This research is expected to provide new insights into the pathogenesis of Keshan disease.
- The findings may lead to the development of novel diagnostic markers and therapeutic strategies.
- Understanding the gut microbiome's role is crucial for advancing Keshan disease management.
Background:
Keshan disease is an endemic cardiomyopathy of undefined causes. Being involved in the unclear pathogenesis of Keshan disease, a clear diagnosis, and effective treatment cannot be initiated. However, the rapid development of gut flora in cardiovascular disease combined with omics and big data platforms may promote the discovery of new diagnostic markers and provide new therapeutic options. This study aims to identify biomarkers for the early diagnosis and further explore new therapeutic targets for Keshan disease.
Methods:
This cohort study consists of two parts. Though the first part includes 300 participants, however, recruiting will be continued for the eligible participants. After rigorous screening, the blood samples, stools, electrocardiograms, and ultrasonic cardiogram data would be collected from participants to elucidate the relationship between gut flora and host. The second part includes a prospective follow-up study for every 6 months within 2 years. Finally, deep mining of big data and rapid machine learning will be employed to analyze the baseline data, experimental data, and clinical data to seek out the new biomarkers to predict the pathogenesis of Keshan disease.
Discussion:
Our study will clarify the distribution of gut flora in patients with Keshan disease and the abundance and population changes of gut flora in different stages of the disease. Through the big data platform analyze the relationship between environmental factors, clinical factors, and gut flora, the main factors affecting the occurrence of Keshan disease were identified, and the changed molecular pathways of gut flora were predicted. Finally, the specific gut flora and molecular pathways affecting Keshan disease were identified by metagenomics combined with metabonomic analysis.
Trial Registration:
ChiCTR1900026639. Registered on 16 October 2019.
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