Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.8K
Leaky Scanning02:28

Leaky Scanning

5.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K
RNA Interference01:23

RNA Interference

27.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.3K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

464
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
464
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

48.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
48.6K
Experimental RNAi02:15

Experimental RNAi

6.9K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lycorine Attenuates Cardiac Fibrosis Through the Regulation of PYK2 Expression and Activity.

Cardiovascular therapeutics·2026
Same author

Inducible T Cell Costimulator Ligand and Inducible T Cell Costimulator Stratification Identify Dichotomous Tumor Microenvironment and Guide Chemo-Immunotherapy in Small Cell Lung Cancer.

MedComm·2026
Same author

Accurate, scalable and cross-platform cell identification for high-resolution spatial transcriptomics.

Nature genetics·2026
Same author

Integrative Analyses Identify a cGAS-STING Pathway-Driven Signature With Context-Dependent Roles in Systemic Lupus Erythematosus.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Surgery after induced anti-PD-L1 therapy and chemotherapy for stage I‒III small-cell lung cancer: a phase 2 trial (LungMate-005).

Cell discovery·2025
Same author

Spatiotemporal analyses of the pan-cancer single-cell landscape reveal widespread profibrotic ecotypes associated with tumor immunity.

Nature cancer·2025

Related Experiment Video

Updated: Nov 30, 2025

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
12:43

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

12.6K

NSUN5 Facilitates Viral RNA Recognition by RIG-I Receptor.

Boyue Sun1, Haoyang Zeng1, Jiaqian Liang1

  • 1State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China.

Journal of Immunology (Baltimore, Md. : 1950)
|November 12, 2020
PubMed
Summary

NSUN5 potentiates the RIG-I innate immune pathway, enhancing antiviral responses against RNA viruses. NSUN5 deficiency impairs viral recognition, increasing susceptibility to infection.

More Related Videos

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
06:44

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

8.2K
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

11.3K

Related Experiment Videos

Last Updated: Nov 30, 2025

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
12:43

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

12.6K
Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
06:44

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

8.2K
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

11.3K

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • The RIG-I receptor is crucial for detecting RNA viruses and initiating innate immune responses.
  • The precise mechanisms regulating RIG-I signaling strength are not fully elucidated.

Purpose of the Study:

  • To investigate the role of NSUN5 in the RIG-I innate immune signaling pathway.
  • To understand how NSUN5 influences the host's response to RNA virus infection.

Main Methods:

  • Investigated NSUN5's effect on RIG-I signaling using cell-based assays.
  • Analyzed RNA virus proliferation in NSUN5-deficient and wild-type models.
  • Utilized mouse models to assess susceptibility to RNA virus infection.
  • Performed biochemical assays to determine the interaction between NSUN5, viral RNA, and RIG-I.

Main Results:

  • NSUN5 was identified as a potentiator of the RIG-I innate signaling pathway.
  • NSUN5 deficiency led to increased RNA virus proliferation and reduced induction of antiviral genes.
  • NSUN5-deficient mice exhibited heightened susceptibility to RNA virus infection.
  • NSUN5 directly binds to viral RNA and RIG-I, enhancing dsRNA recognition.

Conclusions:

  • NSUN5 acts as a novel RIG-I coreceptor, significantly contributing to the restriction of RNA virus infection.
  • This study elucidates a new mechanism by which the innate immune system combats viral pathogens.