Related Experiment Video
Updated: Nov 30, 2025
07:47
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
Published on: September 15, 2023
2.0K
Rab11a Is Overexpressed in Gastric Cancer and Regulates FAK/AKT Signaling
1Department of Pathology, College of Basic Medical Science, China Medical University, The First Affiliated Hospital of China Medical University, Shenyang, China.
Journal of Oncology
|November 12, 2020
Summary
Rab11a protein is upregulated in human gastric cancer, promoting cell growth, invasion, and cisplatin resistance. Its dysregulation may occur through the FAK/AKT signaling pathway, offering potential therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Rab11a dysregulation is linked to various cancers.
- Limited research exists on Rab11a's role in human gastric cancer.
Purpose of the Study:
- To investigate Rab11a protein expression in human gastric cancer.
- To elucidate Rab11a's function in gastric cancer cell proliferation, invasion, and chemoresistance.
Main Methods:
- Examined Rab11a protein expression in gastric cancer tissues and cell lines.
- Utilized plasmid transfection and siRNA to manipulate Rab11a levels in MGC803 and AGS cells.
- Assessed cell proliferation, invasion, apoptosis, and mitochondrial membrane potential.
- Analyzed protein expression via Western blot and FAK inhibition.
Main Results:
- Rab11a was upregulated in 49/108 gastric cancers, correlating with advanced stage and metastasis.
- Rab11a overexpression enhanced cell growth, invasion, and cisplatin resistance, while suppressing apoptosis.
- Rab11a depletion decreased proliferation, invasion, and promoted apoptosis.
- Rab11a influenced MMP2, cyclin D1, Bcl-2, p-FAK, and p-AKT levels, potentially via the FAK/AKT pathway.
Conclusions:
- Rab11a is upregulated in human gastric cancer and promotes key oncogenic processes.
- Rab11a plays a significant role in gastric cancer progression and chemoresistance.
- The FAK/AKT pathway is implicated in Rab11a-mediated effects on gastric cancer cells.
More Related Videos
Related Concept Videos
03:03mTOR Signaling and Cancer Progression
4.3K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.3K
01:22PI3K/mTOR/AKT Signaling Pathway
4.8K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.8K
02:23Abnormal Proliferation
4.9K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
01:07MAPK Signaling Cascades
7.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.4K
01:17Intracellular Signaling Affects Focal Adhesions
3.3K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.3K
01:19Interactions Between Signaling Pathways
6.9K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.9K