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A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
Etai Sapoznik1,2, Bo-Jui Chang1, Jaewon Huh1,2
1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.
Elife
|November 12, 2020
Summary
A new oblique plane microscope (OPM) offers enhanced resolution and usability for biological imaging. This versatile microscope enables high-quality visualization of cellular processes and complex biological systems.
Area of Science:
- Biophysics
- Cell Biology
- Microscopy
Background:
- Traditional light-sheet microscopy has limitations in resolution, field of view, and usability.
- Previous oblique plane microscope (OPM) variants required further optimization for advanced biological imaging applications.
Purpose of the Study:
- To develop an improved oblique plane microscope (OPM) with enhanced resolution, field of view, and user-friendliness.
- To demonstrate the OPM's capability for high-resolution imaging of diverse biological processes and systems.
Main Methods:
- Development of a novel OPM incorporating a bespoke glass-tipped tertiary objective.
- Utilizing high numerical aperture optics for superior lateral and axial resolution.
- Testing the OPM's performance across various biological samples and dynamic phenomena.
Main Results:
- Achieved lateral and axial resolutions comparable to lattice light-sheet microscopy.
- Enabled high-resolution imaging of clathrin-mediated endocytosis, vimentin, endoplasmic reticulum, and Natural Killer-mediated cytotoxicity.
- Successfully imaged challenging phenomena including cell migration in microfluidics, subcellular photoactivation, and large-scale tissue sections.
Conclusions:
- The enhanced OPM provides a user-friendly and versatile platform for advanced biological imaging.
- This OPM achieves high-resolution imaging performance, rivaling specialized techniques.
- The microscope facilitates the study of complex biological dynamics previously difficult to observe.
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