¹⁵ N-carnitine, a novel endogenous hyperpolarized MRI probe with long signal lifetime

The purpose of this study was to investigate hyperpolarization and in vivo imaging of [¹⁵ N]carnitine, a novel endogenous MRI probe with long signal lifetime.

L-[¹⁵ N]carnitine-d₉ was hyperpolarized by the method of dynamic nuclear polarization followed by rapid dissolution. The T₁ signal lifetimes were estimated in aqueous solution and in vivo following intravenous injection in rats, using a custom-built dual-tuned ¹⁵ N/¹ H RF coil at 4.7 T. ¹⁵ N chemical shift imaging and ¹⁵ N fast spin-echo images of rat abdomen were acquired 3 minutes after [¹⁵ N]carnitine injection.

Estimated T₁ times of [¹⁵ N]carnitine at 4.7 T were 210 seconds (in H₂ O) and 160 seconds (in vivo), with an estimated polarization level of 10%. Remarkably, the [¹⁵ N]carnitine coherence was detectable in rat abdomen for 5 minutes after injection for the nonlocalized acquisition. No downstream metabolites were detected on localized or nonlocalized ¹⁵ N spectra. Diffuse liver enhancement was detected on ¹⁵ N fast spin-echo imaging 3 minutes after injection, with mean hepatic SNR of 18 ± 5 at a spatial resolution of 4 × 4 mm.

This study showed the feasibility of hyperpolarizing and imaging the biodistribution of HP [¹⁵ N]carnitine.