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Updated: Nov 30, 2025
Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets
Published on: February 10, 2020
Diagnostic and therapeutic biomarkers for Alzheimer's disease in human-derived platelets
Jae-Woong Min1, Jina Lee2, Hui-Jin Mun2
1Biorchestra Co. Ltd., Techno4-ro 17, Daejeon, 34013, South Korea.
Background:
Diagnosis of current Alzheimer's disease (AD) is difficult even for medical specialists, and there is no clear biomarker. Also, aging is highly related to the onset of AD.
Objectives:
The purpose of this study is to screen miRNA as an aging-considered biomarker for AD treatment and diagnosis.
Methods:
The patient group for this study was divided into a young normal, old normal, or AD group. We developed a method of discovering sequentially expressed miRNAs to distinguish miRNAs that were sequentially expressed in the three groups.
Results:
Sequentially expressed miRNAs correlated highly with the patient's age, and most showed expression patterns that distinguished young, old, and AD. Specifically, the miRNA expression we found showed similar patterns in the brains of patients with AD. Among the selected miRNAs, one set derived from the same precursor: The expression of miR-150 was a disease- and age-specific downregulation in both 3p and 5p forms, and the precursor also had the same pattern. We named that triple matching. Also, the found miR-150 precursor had AD-specific miRNA-imbalance characteristics.
Conclusions:
We developed a novel AD diagnostic method using triple matching and miRNA-imbalance. The triple matching and miRNA imbalance-based relative ratio diagnosis method we developed will be very powerful in resolving the challenges of absolute diagnostic quantification based on biomarker expression. Also, our research results suggest the possibility of a treatment target for AD.
Insights
Researchers identified specific microRNAs (miRNAs) that change with age and Alzheimer's disease (AD). This discovery offers a new way to diagnose AD and may lead to future treatments.
Area of Science:
- Biomarker discovery
- Neuroscience
- Molecular biology
Background:
- Alzheimer's disease (AD) diagnosis is challenging due to a lack of clear biomarkers.
- Aging is a significant risk factor for AD onset.
Purpose of the Study:
- To screen microRNAs (miRNAs) as potential biomarkers for AD, considering age-related changes.
- To identify novel diagnostic and therapeutic targets for AD.
Main Methods:
- Developed a method to identify sequentially expressed miRNAs in young normal, old normal, and AD patient groups.
- Analyzed miRNA expression patterns related to age and AD status.
Main Results:
- Identified specific miRNAs that correlate with age and differentiate between young, old, and AD groups.
- Discovered that miR-150 (both 3p and 5p forms and its precursor) shows disease- and age-specific downregulation in AD patients.
- Found that the miR-150 precursor exhibits AD-specific miRNA-imbalance characteristics, termed 'triple matching'.
Conclusions:
- Developed a novel AD diagnostic method based on 'triple matching' and miRNA imbalance.
- This relative ratio diagnostic approach addresses challenges in absolute biomarker quantification.
- The findings suggest potential new therapeutic targets for Alzheimer's disease.
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