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Related Experiment Videos

Postembedding immunoelectron microscopy using protein A-gold.

M M Silver1, S A Hearn

  • 1Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.

Ultrastructural Pathology
|January 1, 1987
PubMed
Summary
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Immunogold techniques identify antigens in tissues while preserving ultrastructure. This method works for many antigens that survive standard tissue processing procedures.

Area of Science:

  • Electron microscopy
  • Immunohistochemistry
  • Biotechnology

Background:

  • Immunogold techniques are crucial for visualizing antigen localization in cells and tissues.
  • Preserving tissue ultrastructure is essential for accurate interpretation of antigen distribution.
  • Standard fixation and embedding procedures can potentially denature antigens.

Purpose of the Study:

  • To highlight the advantages of immunogold techniques for ultrastructural antigen identification.
  • To discuss the applicability of immunogold techniques across a wide range of antigens.
  • To emphasize the compatibility of immunogold methods with standard tissue preparation.

Main Methods:

  • Utilizing immunogold labeling for antigen detection.
  • Employing standard electron microscopy fixation and embedding protocols (fixation, osmication, dehydration, epoxy resin embedding).

Related Experiment Videos

  • Assessing antigen integrity after harsh processing steps.

    • Main Results:

    • Immunogold techniques successfully identify antigens while maintaining optimal tissue ultrastructure.
    • A broad spectrum of antigens have been shown to withstand the denaturing effects of fixation, osmication, dehydration, and epoxy embedding.
    • The repertoire of antigens compatible with immunogold labeling is continuously expanding.

    Conclusions:

    • Immunogold techniques provide a powerful tool for correlative ultrastructural and antigenic analysis.
    • The robustness of many antigens to standard processing enables widespread application of immunogold methods.
    • Further research will likely identify additional antigens suitable for this technique.