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Updated: Nov 30, 2025
12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
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Twelve quick steps for genome assembly and annotation in the classroom.
Hyungtaek Jung1,2, Tomer Ventura3, J Sook Chung4
1School of Biological Sciences, The University of Queensland, St Lucia, Queensland, Australia.
Plos Computational Biology
|November 12, 2020
Summary
This guide presents 12 essential steps for eukaryotic genome sequencing and de novo assembly projects. It offers broadly applicable guidelines for researchers, covering DNA extraction to data sharing for better species biology understanding.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- Genome sequencing and assembly are now accessible to individual research groups.
- Third-generation sequencing technologies offer advanced genomic capabilities.
- Challenges remain for aquatic species due to genome size and complexity.
Purpose of the Study:
- Provide a 12-step guideline for initiating and completing genome projects.
- Offer broadly applicable and sustainable methods for genome assembly and annotation.
- Address challenges in sequencing and annotating complex eukaryotic genomes.
Main Methods:
- Review of DNA extraction protocols and sequencing platform selection.
- Discussion of bioinformatics pipelines for structural and functional annotation.
- Guidance on community building, data management, and FAIR data principles.
Main Results:
- A comprehensive 12-step framework for genome projects.
- Practical advice on selecting sequencing technologies and library preparation.
- Strategies for effective data management and public data sharing.
Conclusions:
- High-quality genome assembly and annotation are crucial for understanding species biology.
- The presented guidelines facilitate successful genome projects for diverse species.
- Adherence to FAIR principles ensures data accessibility and reusability for the scientific community.
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