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Related Experiment Video

Updated: Nov 30, 2025

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue
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A pH-Adjustable Tissue Clearing Solution That Preserves Lipid Ultrastructures: Suitable Tissue Clearing Method for

Shintaro Fumoto1, Eriko Kinoshita1, Keisuke Ohta2

  • 1Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8501, Japan.

Pharmaceutics
|November 13, 2020
PubMed
Summary

Seebest is a novel pH-adjustable tissue clearing solution that enables rapid, 3D multicolor imaging while preserving lipid ultrastructures. This method is ideal for visualizing biological events and evaluating drug delivery systems.

Keywords:
adult animalbiological imaginggene deliveryliposomemicroscopythree-dimensional imagingtissue optical clearing

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Area of Science:

  • Biotechnology
  • Microscopy
  • Histology

Background:

  • Visualizing complex biological events and states in 3D is crucial for biological research but remains challenging.
  • Existing tissue clearing techniques often struggle with preserving fine details like lipid ultrastructures.

Purpose of the Study:

  • To develop a novel, pH-adjustable tissue clearing solution (Seebest) for enhanced 3D multicolor imaging.
  • To assess the solution's efficacy in preserving lipid ultrastructures and visualizing biological samples.

Main Methods:

  • A pH-adjustable tissue clearing solution, Seebest, was developed using polyethylenimine and urea.
  • The solution's performance was evaluated for its clearing ability, lipid preservation, and compatibility with fluorescent dyes.
  • Applications included visualizing blood vessels, doxorubicin distribution, oxidative stress, and liposomes in tissues.

Main Results:

  • Seebest demonstrated rapid and efficient tissue clearing for multiple tissues within hours.
  • The solution effectively preserved lipid ultrastructures at an electron microscopy level.
  • Optimized pH adjustment maximized fluorescent intensity and minimized dye leakage, enabling clear visualization of biological components and drug delivery systems.

Conclusions:

  • Seebest offers a rapid, pH-adjustable tissue clearing method that preserves lipid ultrastructures.
  • The solution facilitates 3D multicolor imaging for diverse biological applications, including drug delivery system evaluations.