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Updated: Nov 30, 2025

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition.

Claudia Riccardi^1,2, Ettore Napolitano¹, Domenica Musumeci^1,3

¹Department of Chemical Sciences, University of Naples Federico II, via Cintia 21, I-80126 Naples, Italy.

Molecules (Basel, Switzerland)

|November 13, 2020

Summary

Multivalent aptamers, inspired by nature, offer enhanced binding affinity and selectivity for biological targets. DNA-based dimeric and multimeric aptamers show promise as therapeutic agents.

Keywords:

G-quadruplex aptamer design dimerization molecular recognition multivalency protein target therapy

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Published on: January 14, 2020

Area of Science:

Biochemistry and Molecular Biology
Biotechnology and Nanotechnology
Medicinal Chemistry

Background:

Multivalent interactions in biological systems enhance binding affinity and target selectivity compared to monovalent interactions.
Nucleic acid aptamers, particularly DNA-based ones, are versatile building blocks for creating multivalent systems due to ease of modification and assembly.
Previous research has focused on developing dimeric/multimeric aptamers to improve target binding and therapeutic efficacy.

Purpose of the Study:

To review recent advancements in the design and development of dimeric and multimeric DNA-based aptamers.
To highlight aptamers, including G-quadruplex (G4) forming structures, targeting key proteins in pathological processes.
To summarize their improved performance in binding affinity and therapeutic activities.

Main Methods:

Review of literature on the design and construction of dimeric and multimeric DNA aptamers.
Analysis of aptamer constructs, including those forming G-quadruplex (G4) structures.
Evaluation of aptamer performance in terms of binding affinity and therapeutic activity enhancement.

Main Results:

Dimeric and multimeric DNA aptamers, including G4 aptamers, have been successfully designed and developed.
These constructs demonstrate enhanced binding affinity and improved therapeutic activities.
Applications include anti-inflammatory, antiviral, anticoagulant, and anticancer agents.

Conclusions:

Multivalent DNA aptamers represent a promising strategy for developing highly effective therapeutic agents.
The design of dimeric and multimeric aptamers, especially G4 structures, leads to superior target recognition and therapeutic outcomes.
Continued growth in the development and application of these advanced aptamer systems is anticipated.