Single-shot wavelength-selective quantitative phase microscopy by partial aperture imaging and...

Area of Science:

• Biomedical Optics
• Microscopy
• Phase Imaging

Background:

• Quantitative phase microscopy (QPM) is crucial for label-free biological imaging.
• Conventional QPM often requires complex setups and multiple measurements.
• Limitations exist in speed and sensitivity for dynamic biological processes.

Purpose of the Study:

• To develop a single-shot, wavelength-selective QPM method.
• To enhance spatiotemporal phase sensitivity and imaging speed.
• To provide a robust alternative to conventional multi-wavelength digital holography.

Main Methods:

• Utilizing annular white-light illumination.
• Implementing polarization-phase-division multiplexing.
• Employing parallel phase-shifting techniques for single-shot acquisition.

Main Results:

• Demonstrated wavelength-selective quantitative phase imaging.
• Achieved high spatiotemporal phase sensitivity.
• Successfully reconstructed phase images in a single shot per wavelength.
• Validated performance on gratings, cells, and tissues.

Conclusions:

• The proposed method offers significant advantages over traditional QPM.
• It enables rapid, high-sensitivity phase imaging without mechanical scanning.
• This technique is suitable for diverse applications in biological and material sciences.