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Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon
J Steen Hoyer1, Vincent N Fondong2, Mary M Dallas3
1Department of Ecology, Evolution, and Natural Resources, Rutgers University, New Brunswick, New Jersey, USA j.s.hoyer@wustl.edu duffy@sebs.rutgers.edu.
Microbiology Resource Announcements
|November 13, 2020
Summary
Deep sequencing of infectious clones for African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) revealed significant divergence in ACMV sequences. All data and clones are publicly available for research.
Area of Science:
- Plant virology
- Molecular biology
- Genomics
Background:
- Bipartite begomoviruses, such as ACMV and EACMCV, are significant pathogens of cassava.
- Infectious clones are crucial tools for studying begomovirus replication and pathogenicity.
- Accurate sequence data of infectious clones is essential for reproducible research.
Purpose of the Study:
- To deeply sequence and characterize widely used infectious clones of ACMV and EACMCV.
- To assess the sequence integrity and potential divergence of these essential research tools.
- To make sequencing data and clones publicly available.
Main Methods:
- Deep sequencing of four plasmid DNA samples representing two infectious clones each of ACMV and EACMCV.
- Bioinformatic analysis to generate consensus sequences from raw sequencing reads.
- Public deposition of raw reads, consensus sequences, and infectious clones.
Main Results:
- Deep sequencing provided high-quality consensus sequences for the infectious clones.
- Significant sequence divergence was observed between the sequenced ACMV clones and previously published sequences.
- The EACMCV sequences were consistent with published data.
Conclusions:
- The characterized ACMV infectious clones exhibit notable sequence divergence, highlighting the need for verification.
- Public availability of raw reads, consensus sequences, and clones facilitates transparency and further research.
- This study provides essential, validated resources for the begomovirus research community.
