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Updated: Nov 30, 2025
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Published on: August 30, 2018
Rapid, high-throughput method for the quantification of thyroid hormones in rat blood serum using
Charles R Powley1,2, Jinlan Dong1, Bethany R Hannas1
1R&D Center, Corteva Agriscience, PO Box 30, 1090 Elkton Rd., Bldg. 320, Newark, DE 19714, USA.
Abstract:
Aim: Numerous guideline studies required for regulatory toxicity testing now include the measurement of the thyroid hormones 3,3',5-triiodo-L-thyronine (T3) and L-thyroxine (T4) in blood serum from rodents. A rapid, high-throughput method for the determination of the thyroid hormones T4 and T3 is reported. Materials & methods: Sample preparation is done using a 96-well microtiter plate format. Stable isotope analogs of both hormones are used as internal standards for study and quality control samples. Results & conclusion: The validated quantification levels are T3: 10 pg/ml and T4: 1 ng/ml, with CVs of <10% at the limit of quantification and up to 50*limit of quantification. The use of isotope analog internal standards eliminates the need for quantitative transfers and complete evaporations.
