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Pigment production in group B streptococci.
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|August 1, 1977
Summary
Most hemolytic group B streptococci produce a yellow-orange pigment. Medium composition and trypsin affect pigment production, with distinct optical density peaks observed in pigmented strains.
Area of Science:
- Microbiology
- Bacteriology
- Biochemistry
Background:
- Group B Streptococcus (GBS) is a significant human pathogen.
- Certain GBS strains exhibit pigment production, but its characteristics are not fully understood.
Purpose of the Study:
- To investigate the pigment production of hemolytic group B streptococci.
- To identify factors influencing pigment formation and characterize the pigment's spectral properties.
Main Methods:
- Culturing GBS strains on Colombia agar and Todd Hewitt broths.
- Assessing pigment production and its inhibition by trypsin.
- Analyzing pigment spectral characteristics using optical density measurements.
Main Results:
- Most hemolytic GBS strains produced a yellow-orange pigment on specific media.
- Pigment production was dependent on broth medium composition.
- Trypsin inhibited pigment formation but did not affect pre-existing pigment.
- Spectrophotometry revealed distinct peaks at 420, 260, and 215 nm for pigmented strains.
Conclusions:
- Hemolytic GBS pigment production is a notable characteristic influenced by media and enzymatic factors.
- The pigment exhibits unique spectral properties, distinguishing it from non-pigmented strains.
- Further research into the pigment's chemical nature and biological role is warranted.