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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Antigen Presenting Cells01:22

Antigen Presenting Cells

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The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
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Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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Related Experiment Video

Updated: Nov 30, 2025

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
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Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

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Some Like It Sweet: Dendritic Cells Add Sugar to Their T(ea).

A C Kohlgruber1, C I Wang2, S J Elledge3

  • 1Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Genetics, Harvard University Medical School, Boston, MA 02115, USA.

Cell
|November 13, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed FucoID, a new platform to label and capture specific T cells. This technology aids in studying anti-tumor immune responses by isolating tumor-specific T cells.

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Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
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Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
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Area of Science:

  • Immunology
  • Biochemistry
  • Molecular Biology

Background:

  • Identifying and isolating specific T cells is crucial for understanding immune responses, particularly in cancer.
  • Current methods for T cell isolation can be challenging and may not capture all relevant cell populations.

Purpose of the Study:

  • To introduce FucoID, a novel glycosyltransferase-mediated tagging platform.
  • To demonstrate the utility of FucoID for biochemically labeling and capturing antigen-specific T cells.
  • To characterize tumor-specific T cells in preclinical cancer models.

Main Methods:

  • Development of FucoID, a platform utilizing glycosyltransferase activity for T cell labeling.
  • Application of FucoID to label and capture antigen-specific T cells.
  • Isolation and characterization of CD8+ and CD4+ T cells from murine tumor models.

Main Results:

  • Successful biochemical labeling and capture of antigen-specific T cells using FucoID.
  • Isolation and characterization of tumor-specific CD8+ and CD4+ T cells.
  • Demonstration of FucoID's efficacy in preclinical murine tumor models.

Conclusions:

  • FucoID is a powerful new tool for the biochemical isolation and study of antigen-specific T cells.
  • This platform enhances the understanding of anti-tumor immune responses.
  • FucoID shows significant promise for immunological research and therapeutic development.