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Updated: Nov 30, 2025
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
Comparative microRNAs expression profiles analysis during embryonic development of common carp, Cyprinus carpio
Lanmei Wang1, Feibiao Song2, Haoran Yin2
1Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, Jiangsu, China; Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi 214081, Jiangsu, China.
This study identifies key microRNAs (miRNAs) crucial for common carp embryonic development. These findings offer new insights into miRNA-regulated gene expression during fish embryogenesis.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- MicroRNAs (miRNAs) are vital regulators of gene expression in biological processes.
- Information on miRNA roles in common carp (Cyprinus carpio) embryonic development is scarce.
Purpose of the Study:
- To comprehensively identify and characterize microRNAs (miRNAs) during common carp embryonic development.
- To investigate the expression profiles and roles of differentially expressed miRNAs (DEMs) in key developmental stages.
Main Methods:
- Time-series small RNA sequencing was performed on six distinct embryonic stages of common carp.
- Bioinformatic analyses were used to identify known and novel miRNAs and screen for differentially expressed miRNAs (DEMs).
Main Results:
- A total of 2565 miRNAs were identified across the developmental stages.
- 68 of 204 differentially expressed miRNAs (DEMs) overlapped with stage-specific miRNAs, with 15 known miRNAs showing significant roles.
- Expression profiling revealed dynamic miRNA fluctuations and identified involvement in pathways like hippo signaling and Wnt.
Conclusions:
- This research provides a foundational understanding of miRNA involvement in common carp embryogenesis.
- The identified miRNAs and pathways offer novel insights into miRNA-mediated gene regulation during fish embryonic development.
