Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide CRISPR-Cas9-based screening revealed the role of ubiquitin ligase TRIM25 in ribosome degradation via ribophagy.

Autophagy·2026
Same author

Unlocking ferroptosis heterogeneity: ATF4 versus SREBF transcriptional programs.

Cell death discovery·2026
Same author

Genome sequence data of the plant growth-promoting rhizobacterium <i>Pseudomonas inefficax</i> MG-2.

Microbiology resource announcements·2026
Same author

Chromosome-level genome assembly of the sponge Halisarca dujardinii.

Scientific data·2026
Same author

An Initial Assessment of Rabbit Cornea as a Biomarker of Trace-Element Load in Commercial Animal Production.

Metabolites·2026
Same author

[Next-generation pharmaceutically acceptable vehicles for eye drops (preliminary report)].

Vestnik oftalmologii·2026

Related Experiment Video

Updated: Nov 30, 2025

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression

Published on: March 5, 2013

35.9K

Development of a Tet-On Inducible Expression System for the Anhydrobiotic Cell Line, Pv11.

Shoko Tokumoto1, Yugo Miyata2, Kengo Usui3

  • 1Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8562, Japan.

Insects
|November 14, 2020
PubMed
Summary

Researchers developed an inducible expression system for Pv11 cells, the only desiccation-tolerant cell line. This system enables controlled gene expression in cells preserved in a dry state, opening possibilities for producing complex proteins.

Keywords:
Polypedilum vanderplankiTet-On systemanhydrobiosisdesiccation toleranceinsect cellsminimal promoter

More Related Videos

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.9K
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

11.2K

Related Experiment Videos

Last Updated: Nov 30, 2025

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression

Published on: March 5, 2013

35.9K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.9K
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

11.2K

Area of Science:

  • Insect cell line biotechnology
  • Molecular biology
  • Gene expression systems

Background:

  • The Pv11 cell line from *Polypedilum vanderplanki* is unique for its tolerance to complete desiccation.
  • Previous work established constitutive gene expression in Pv11 cells.

Purpose of the Study:

  • To identify an effective minimal promoter for Pv11 cells.
  • To establish a Tet-On inducible expression system in Pv11 cells.
  • To demonstrate the utility of this system for expressing functional proteins.

Main Methods:

  • Luciferase reporter assays were used to identify a minimal promoter fragment.
  • The Tet-On system was applied to Pv11 cells using the identified minimal promoter.
  • Expression and activity of reporter genes (AcGFP1) and a functional enzyme (AMV RTα) were verified.

Main Results:

  • A 202 bp deletion fragment of the 121-promoter was identified as an effective minimal promoter for Pv11 cells.
  • The Tet-On inducible expression system was successfully established in Pv11 cells.
  • Functional expression of AcGFP1 and avian myeloblastosis virus reverse transcriptase alpha subunit (AMV RTα) was achieved.

Conclusions:

  • An inducible gene expression system has been established in desiccation-tolerant Pv11 cells.
  • This system allows for controlled gene expression in cells that can be stored dry.
  • Potential applications include the production of large and complex proteins in a stable cell line.