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Updated: Nov 30, 2025

Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

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Seeing Is Believing: Visualizing Circular RNAs.

Pruthvi Raj Bejugam1, Aniruddha Das1,2, Amaresh Chandra Panda1

  • 1Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha 751023, India.

Non-Coding RNA
|November 14, 2020
PubMed
Summary
This summary is machine-generated.

Advancements in RNA sequencing have identified numerous circular RNAs (circRNAs), but their functions are largely unknown. This review explores advanced RNA imaging techniques to visualize circRNAs and their regulators in cells.

Keywords:
BaseScopeFISHRNA-binding proteinaptamercircRNAlocalizationmiRNA

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Hundreds of thousands of circular RNAs (circRNAs) have been identified in humans using advanced RNA sequencing techniques.
  • The physiological functions of most circRNAs remain largely unexplored, despite their modulation of cellular processes by interacting with proteins and microRNAs.
  • Challenges in studying circRNAs include their low abundance and sequence similarity to linear RNAs, hindering understanding of their biogenesis, transport, and regulatory mechanisms.

Purpose of the Study:

  • To review current and proposed RNA imaging techniques for visualizing circRNAs in cells.
  • To discuss the benefits and limitations of various single-molecule resolution imaging methods.
  • To highlight the importance of visualizing circRNAs and their molecular regulators for functional studies.

Main Methods:

  • Review of existing RNA imaging techniques: single-molecule RNA fluorescence in situ hybridization (smFISH) and BaseScope for fixed cells.
  • Discussion of live-cell imaging techniques using fluorescent RNA aptamers.
  • Exploration of potential future techniques including molecular beacons, multiply labeled tetravalent RNA imaging probes, and Cas-derived systems.

Main Results:

  • Existing techniques like smFISH and BaseScope allow for circRNA visualization in fixed cells with varying degrees of resolution and specificity.
  • Fluorescent RNA aptamers offer potential for live-cell imaging of circRNAs, enabling dynamic studies.
  • Novel approaches are proposed to overcome current limitations in circRNA visualization.

Conclusions:

  • Advanced RNA imaging techniques are crucial for elucidating the functions of circRNAs.
  • Visualizing circRNAs at single-molecule resolution is essential for understanding their interactions with cellular factors.
  • Future development of imaging technologies will significantly advance circRNA research.