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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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β-Carboline-Based pH Fluorescent Probe and Its Application for Monitoring Enzymatic Ester Hydrolysis
Rong Huang1,2, Xian-Dong Zhang1, Xing Wu1
1School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China.
Chemistry & Biodiversity
|November 14, 2020
Abstract:
A novel pH-activatable fluorescent probe, 1-(propan-2-yl)-9H-pyrido[3,4-b]indole-3-carboxylic acid (L-1), based on β-carboline derivatives, has been developed, which displays significant fluorescent response toward pH variation with high selectivity, good photo-stability and favorable pKa value. Moreover, L-1 can dynamically monitor the release of protons during ester hydrolysis reaction in consistent with enzymatic kinetics manner.

