Evaluation of vitamin A gut loading in black soldier fly larvae (Hermetia illucens)

Black soldier fly (BSF) larvae are potentially an excellent source of calcium for insectivores; however, previous studies have identified that they lack appreciable amounts of fat-soluble vitamins (A, D₃ , and E). To make BSF larvae a more complete food item, fat-soluble vitamins should either be provided via gut loading or with a multivitamin dusting supplement. The purpose of this study is to identify factors associated with gut loading vitamin A into BSF larvae and to develop feeding recommendations for a more consistent gut-loading process. Factors that were addressed include the vitamin A concentration added to the diet, length of time given to gut load, moisture content of the diet, and density of larvae during feeding. Diets and larvae were analyzed for vitamin A concentration using high-performance liquid chromatography. Larval vitamin A concentrations increased in a nonlinear fashion with increasing dietary vitamin A. Length of time (F = 150.818, p < .001), moisture content of the diet (F = 41.436, p < .001), and larval density (F = 78.407, p < .001) were all found to be significant factors contributing to the larvae's gut-loading capacity. On the basis of our results and vitamin A recommendations from the National Resource Council for rats and poultry, gut-loading recommendations for BSF larvae when fed to insectivorous reptiles and amphibians are as follows: vitamin A concentration of diet between 16,000 and 20,000 mcg retinol equivalents/kg, gut-loading time period of 24 h, moisture content of the diet approximately 60%, and larval density between 0.1 and 1 larvae per each gram of moist substrate.