Incidence of metal-based nanoparticles in the conventional wastewater treatment process

Metal-based nanoparticles (NPs) can be found in wastewater streams, which are significant pathways for the release of NPs to the environment. Determination of the NPs concentration in wastewater streams is important for performing appropriate ecotoxicological evaluations. The aim of this work was to determine the incidence of NPs from 13 different elements throughout the wastewater treatment process by using single particle inductively coupled plasma mass spectrometry (spICP-MS). The incidence was determined in samples of the influent, post-primary treatment and effluent of the activated sludge process, as well as in the reclaimed water of a full-scale wastewater treatment plant (WWTP). In addition, concentration of NPs was determined in the waste activated sludge and in the anaerobic digester. The concentration of metal-based NPs in the influent wastewater were between 1,600 and 10,700 ng/L for elements such as Ti, Fe, Ce, Mg, Zn and Cu, while that for Ni, Al, Ag, Au, Co and Cd was below 100 ng/L. Concentrations in reclaimed water ranged between 0.6 and 721 ng/L, ranked as Mg > Ti > Fe > Cu > Ni > Ce > Zn > Mn > Al  > Co > Ag > Cd  > Au. Results indicated that the activated sludge process and reclaimed water system removed 84-99% of natural and engineered metal-based NPs from influent to reclaimed water, except for Mg, Ni and Cd where the removal ranged from 70 to 78%. The highest concentrations of NPs were found in the waste activated sludge and anaerobic sludge, ranging from 0.5 to 39,900 ng/L. The size distribution of NPs differed in different wastewater streams within the WWTP, resulting in smaller particles in the effluent (20-180 nm) than in the influent (23-233 nm) for most elements. Conversely, NPs were notably larger in the waste activated sludge samples than in the anaerobic sludge or wastewater, since conditions in the secondary treatment lead to precipitation of several metal-based NPs. The incidence of metal-based NPs from 13 elements in wastewater decreased significatively after the conventional wastewater treatment train. However, anaerobic digesters store high NPs concentrations. Hence, the disposal of sludge needs to take this into account to evaluate the risk of the release of NPs to the environment.