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Polyphenol oxidase-based electrochemical biosensors: A review
Paulo A Raymundo-Pereira1, Tiago A Silva2, Fábio R Caetano3
1São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil.
Analytica Chimica Acta
|November 16, 2020
Summary
Electrochemical biosensors using polyphenol oxidases (PPO) offer sensitive detection of phenolic compounds. Nanomaterials enhance enzyme activity and electron transport, paving the way for field analysis and biomedical applications.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Phenolic compounds are vital for health and act as endocrine disruptors.
- Electrochemical biosensors with polyphenol oxidases (PPO) enable on-site detection.
- Current methods require advancements for field applicability.
Purpose of the Study:
- To review electrochemical biosensors utilizing PPOs for phenolic compound detection.
- To highlight the role of nanomaterials in enhancing biosensor performance.
- To discuss market-readiness and challenges for biomedical applications.
Main Methods:
- Literature survey of PPO-based electrochemical biosensors.
- Focus on tyrosinase and laccase enzyme sources (crude extracts, plant tissues).
- Analysis of nanomaterial integration (metallic nanoparticles, carbon nanostructures).
Main Results:
- Nanomaterials significantly improve biosensor sensitivity and robustness.
- Metallic nanoparticles and carbon nanostructures enhance enzyme stability and electron transfer.
- Recent advances focus on synergistic effects between enzymes and nanomaterials.
Conclusions:
- PPO-based electrochemical biosensors show great promise for detecting phenolic compounds.
- Nanomaterial integration is key to developing sensitive and stable biosensors.
- Further development is needed to overcome challenges for commercialization, particularly in biomedical fields.
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